Anti-Flag-Tag Antibody (A50042)

1ug Flag fusion protein+ Primary antibody #T519 dilution at 1: 10,000.

IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb #T519 at a 1: 2000 dilution (blue DAPI, red anti-Flag).

IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1: 5000 1 ) untransfected 293 cell lysate 2 ) transfected 293 cell lysate with Flag-tag fusion protein 3 ) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4 ) IP (transfected 293+anti- Flag mAb+ Protein G agarose) 5 ) IP (transfected 293+Protein G) 6 ) Recombinant protein (E.coli).