Rabbit polyclonal antibody to FKHRL1 (Phospho-Ser253)
Specificity
The antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
97kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C
Synonyms
AF6q21 protein, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1, FOXO3
Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (#21171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (#11157, Lane 3 and 4).