Western Blot - Anti-FKBP52 Antibody [Hi52C] (A305118)
Western blot analysis of human Cell lysates showing detection of FKBP52 protein using Anti-FKBP52 Antibody [Hi52C] (A305118) at 1.5 µg/mL for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human MCF-7 cells (metastatic mammary gland/breast cell line), using Anti-FKBP52 Antibody [Hi52C] (A305118), at 1:1,000. The secondary antibody used was APC Goat Anti-Mouse (red).
Western Blot - Anti-FKBP52 Antibody [Hi52C] (A305118)
Western blot analysis of rat brain, heart, kidney, liver, pancreas, skeletal muscle, spleen, testes, thymus cell lysates showing detection of FKBP52 protein using Anti-FKBP52 Antibody [Hi52C] (A305118) at 1.5 µg/mL for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-FKBP52 Antibody [Hi52C] (A305118) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.