Ficlatuzumab Biosimilar - Anti-HGF Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to HGF.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
Hepatocyte growth factor, Hepatopoietin-A, HPTA, Scatter factor, SF
ELISA plates were pre-coated with Recombinant Human HGF Protein (Fc Tag) (A325006) at 2 µg/ml (100 µl/well) which can bind Ficlatuzumab Biosimilar - Anti-HGF Antibody - BSA and Azide free (A324666) in a linear range of 16-2000 ng/ml. In order to specifically detect Ficlatuzumab Biosimilar - Anti-HGF Antibody - BSA and Azide free (A324666), Mouse Anti-Human Fab Antibody was used as detection antibody.
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