Mouse monoclonal [M5G10] antibody to Fibroblast Growth Factor Receptor 1.
Specificity
Reacts with both alpha (denatured) and beta isoforms. Epitope is within the sequence his241 and val267 between Ig loops II and III. Epitope is masked in undenatured Fibroblast Growth Factor Receptor 1.
Applications
WB, IHC, IP
Dilutions
WB: 1 µg/ml, IHC-P: 10 µg/ml
Reactivity
Human
Immunogen
Recombinant human ectodomain of Fibroblast Growth Receptor 1 beta expressed in E. coli beginning with proline 23; antigen contained NH2-terminal Gly-Ser-Pro-Gly-Ile and COOH-terminal Glu-Phe sequences.
Host
Mouse
Clonality
Monoclonal
Clone ID
M5G10
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
WB detection of Fibroblast Growth Factor Receptor 1 using A304468 at 1 µg/ml. Both the alpha and beta isoforms are detected when recombinantly over-expressed in Sf9 cells.
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