Anti-Fibrillarin Antibody [ARC0506] (A306505)

Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody [ARC0506] (A306505) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody [ARC0506] (A306505) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Confocal imaging of U-2OS cells using Anti-Fibrillarin Antibody [ARC0506] (A306505), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.