Western blot analysis of extracts of various cell lines, using Anti-Fibrillarin Antibody (A89516) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg of Anti-Fibrillarin Antibody (A89516). This Western blot was performed on the immunoprecipitate using Anti-Fibrillarin Antibody (A89516) at a dilution of 1:1000.
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