Confocal immunofluorescent analysis of HeLa cells stained with Anti-Fibrillarin Antibody, at a dilution of 1:10,000, in green, and Anti-Vimentin Antibody (A85424 | 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The Anti-Fibrillarin Antibody stains nucleoli while the Anti-Vimentin Antibody binds to cytoplasmic intermediate filaments.
Western blot analysis of different cell lysates using Anti-Fibrillarin Antibody, at a dilution of 1:5,000, in green,: [Lane 1] protein standard (red), [Lane 2] NIH-3T3, [Lane 3] HEK293, [Lane 4] HeLa, [Lane 5] SH-SY5Y, and [Lane 6] C6 cells. The single strong band at ~35kDa corresponds to the fibrillarin protein.
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