Anti-Fibrillarin Antibody [4A4] (A104318)

Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Anti-Fibrillarin Antibody (1:1,000 | red), in both cases co-stained with Anti-Vimentin Antibody (1:10,000 | green). The blue is DAPI staining of nuclear DNA. The Anti-Fibrillarin Antibody detects fibrillarin protein localized in nucleoli while the Anti-Vimentin Antibody produces strong staining of cytoplasmic intermediate filaments.

Western blot analysis of different tissue and cell line lysates using Anti-Fibrillarin Antibody (1:2,000 | green): [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with Anti-UCHL1 Antibody (1:3,000 | red), revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.