Anti-FHL1 Antibody [ARC1238] (A306871)

Name: Anti-FHL1 Antibody [ARC1238]
Brand: Antibodies.com
SKU: A306871
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1238] antibody to FHL1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-FHL1 Antibody [ARC1238]

Description

Rabbit monoclonal [ARC1238] antibody to FHL1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FHL1 (Q13642).

Sequence

MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1238

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FHL-1, Four and a half LIM domains protein 1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1, SLIM1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-FHL1 Antibody [ARC1238] (A306871)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-FHL1 Antibody [ARC1238] (A306871) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-FHL1 Antibody [ARC1238] (A306871)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-FHL1 Antibody [ARC1238] (A306871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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