Anti-Fbxw7 Antibody (A15027)

Name: Anti-Fbxw7 Antibody
Brand: Antibodies.com
SKU: A15027

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This product is discontinued

Anti-Fbxw7 Antibody (A15027) has been discontinued and is no longer available.

View all Fbxw7 Antibodies.

Specifications

Name

Anti-Fbxw7 Antibody

Description

Rabbit polyclonal antibody to Fbxw7.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150 to 250 of human,.

Sequence

SATPT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

76 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW7, FBX30, hAgo, hCdc4, SEL-10, SEL10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Fbxw7 Antibody (A15027)

Western blot analysis of various lysates, using Anti-Fbxw7 Antibody (A15027) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Fbxw7 Antibody (A15027)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Fbxw7 Antibody (A15027) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Fbxw7 Antibody (A15027)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Fbxw7 Antibody (A15027) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.