Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468)

Name: Anti-Fatty Acid Synthase Antibody [ARC0377]
Brand: Antibodies.com
SKU: A305468
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0377] antibody to Fatty Acid Synthase for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Fatty Acid Synthase Antibody [ARC0377]

Description

Rabbit monoclonal [ARC0377] antibody to Fatty Acid Synthase.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human FASN (P49327).

Sequence

AVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0377

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

273 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FAS, FASN, Type I fatty acid synthase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468)

Western blot analysis of extracts of various cell lines, using Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468)

Immunofluorescence analysis of HeLa cells using Anti-Fatty Acid Synthase Antibody [ARC0377] (A305468) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).