Anti-Fatty Acid Synthase Antibody (A308729)

Western blot analysis of various lysates, using Anti-Fatty Acid Synthase Antibody (A308729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.

Western blot analysis of Rat liver, using Anti-Fatty Acid Synthase Antibody (A308729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.

Western blot analysis of extracts from wild type(WT) and Fatty Acid Synthase (FASN) Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-Fatty Acid Synthase Antibody (A308729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.