Anti-Fascin Antibody [ARC1638] (A306042)

Name: Anti-Fascin Antibody [ARC1638]
Brand: Antibodies.com
SKU: A306042
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1638] antibody to Fascin for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Fascin Antibody [ARC1638]

Description

Rabbit monoclonal [ARC1638] antibody to Fascin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 249-362 of human Fascin/FSCN1 (Q16658).

Sequence

GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1638

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

55 kDa actin-bundling protein, FAN1, FSCN1, HSN, p55, Singed-like protein, SNL

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Fascin Antibody [ARC1638] (A306042)

Western blot analysis of extracts of various cell lines, using Anti-Fascin Antibody [ARC1638] (A306042) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Fascin Antibody [ARC1638] (A306042)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Fascin Antibody [ARC1638] (A306042) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Fascin Antibody [ARC1638] (A306042)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Fascin Antibody [ARC1638] (A306042) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Fascin Antibody [ARC1638] (A306042)

Immunohistochemistry analysis of paraffin-embedded human Hodgkin lymphoma (positive control antibody staining, clone 55k-2) using Anti-Fascin Antibody [ARC1638] (A306042) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.