Anti-Fas Ligand Antibody (A11128)

Name: Anti-Fas Ligand Antibody
Brand: Antibodies.com
SKU: A11128
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Fas Ligand for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Fas Ligand Antibody

Description

Rabbit polyclonal antibody to Fas Ligand.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FasLG (NP_000630.1).

Sequence

GMFQLFHLQKELAELRESTSQMHTASSLEKQIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 ligand, CD95-L, CD95L, Fas antigen ligand, FasL, FASLG, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Fas Ligand Antibody (A11128)

Western blot analysis of extracts of Mouse heart, using Anti-Fas Ligand Antibody (A11128) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5min.

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Immunohistochemistry - Anti-Fas Ligand Antibody (A11128)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Fas Ligand Antibody (A11128) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Fas Ligand Antibody (A11128)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Fas Ligand Antibody (A11128) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Fas Ligand Antibody (A11128)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Fas Ligand Antibody (A11128) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.