Recombinant Anti-Fas Antibody [rB-R18] (A314950)

Anti-Fas Antibody [rB-R18]

Recombinant mouse monoclonal [rB-R18] antibody to Fas.

MAb rB-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

FUNC, Flow Cytometry, IF, IHC-Fr

MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).

Functional Studies, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC: Frozen: 0.5-1 µg/ml

Human

Recombinant human CD95 protein.

Mouse

Monoclonal

rB-R18

kappa

Protein A/G chromatography.

200 µg/ml

38-50 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Apo-1 antigen, Apoptosis-mediating surface antigen FAS, APT1, CD95, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6