Mouse monoclonal [LT95] antibody to Fas (PE-DyLight® 594).
Specificity
The antibody recognizes a functional epitope of Fas molecule.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
HUT-78 human T cell lymphoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
LT95
Isotype
IgG1
Conjugate
PE-DyLight® 594
Purification
This antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human CD95 positive T cells (red-filled) from CD95 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD95 Antibody [LT95] (PE-DyLight 594) (4 µl reagent / 100 µl of peripheral whole blood).
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