Anti-Fas Antibody [ARC0061] (A305713)

Name: Anti-Fas Antibody [ARC0061]
Brand: Antibodies.com
SKU: A305713
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0061] antibody to Fas for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Plasma Membrane Marker

Specifications

Name

Anti-Fas Antibody [ARC0061]

Description

Rabbit monoclonal [ARC0061] antibody to Fas.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Fas (NP_000034.1).

Sequence

EGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0061

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apo-1 antigen, Apoptosis-mediating surface antigen FAS, APT1, CD95, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Fas Antibody [ARC0061] (A305713)

Western blot analysis of extracts of various cell lines, using Anti-Fas Antibody [ARC0061] (A305713) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Fas Antibody [ARC0061] (A305713)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Fas Antibody [ARC0061] (A305713) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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