Fas expression in MOLT4 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Fas Antibody (A82554) at 0.1µg/ml and detected by chemiluminescence.
Fas expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Fas Antibody (A82554) at 10µg/ml and revealed with alkaline phosphatase (AP).
Fas expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Fas Antibody (A82554) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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