Rabbit polyclonal antibody to FAK (Phospho-Tyr925)
Specificity
The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.
Applications
WB, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from 293 cells using FAK (Phospho-Tyr925) Antibody #11123 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).