Anti-Factor VIII Antibody [RFF-VIIIC/8] (A281668)

Anti-Factor VIII Antibody [RFF-VIIIC/8]

Mouse monoclonal [RFF-VIIIC/8] antibody to Factor VIII.

This antibody recognises human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerizes and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. recognises an epitope towards the N-terminus of full-length Factor VIII. It also recognises the 210 kDa, 90 kDa and 40 kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.

WB, ELISA, RIA

ELISA: 1:500 - 1:15,000

Human, Pig

This antibody does not cross react with Mouse, Dog, or Rat

Affinity purified human Factor VIII.

Mouse

Monoclonal

RFF-VIIIC/8

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerizes and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210 kDa, 90 kDa and 40 kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.

AHF, Antihemophilic factor, Coagulation factor VIII, F8, F8C, Procoagulant component