Rabbit polyclonal antibody to ETS1 (Phospho-Thr38)
Specificity
The antibody detects endogenous levels of ETS1 only when phosphorylated at threonine 38.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 38(L-L-T(p)-P-S) derived from Human ETS1 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody #11658.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody #11658 (left)or the same antibody preincubated with blocking peptide (right).
![SDS-PAGE - Anti-ETS1 Antibody [ETS1/1801] - BSA and Azide free (A251676) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251676_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-ETS1 Antibody [ETS1/1801] (A248494) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248494_1.jpg?profile=product_alternative)
![Western Blot - Anti-ETS1 Antibody [ARC0082] (A307447) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307447_1.jpg?profile=product_alternative)
