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Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489)

Western Blot - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.com Immunohistochemistry - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.com Immunofluorescence - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to Estrogen Receptor alpha (phospho Ser167) for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Estrogen Receptor alpha (phospho Ser167) Antibody
Description
Rabbit polyclonal antibody to Estrogen Receptor alpha (phospho Ser167).
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200
Reactivity
Human
Immunogen
A synthetic phosphorylated peptide around S167 of human Estrogen Receptor alpha (ESR1) (NP_000116.2).
Sequence
LASTN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
66 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ER, ER-alpha, ESR, ESR1, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.comEnlarge Image

Western Blot - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489)

Western blot analysis of extracts from MCF7 cells using Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489)

Immunofluorescence analysis of methanol-fixed MCF-7 cells using Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489).
Publishing research using Anti-Estrogen Receptor alpha (phospho Ser167) Antibody (A12489)? Please let us know so that we can list the citation on this page.

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