Western Blot - Anti-Estrogen Receptor alpha (phospho Ser118) Antibody (A12459)
Western blot analysis of extracts of MCF7 cells, using Anti-Estrogen Receptor alpha (phospho Ser118) Antibody (A12459) at 1:1,000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Estrogen Receptor alpha (phospho Ser118) Antibody (A12459). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of methanol-fixed MCF-7 cells using Anti-Estrogen Receptor alpha (phospho Ser118) Antibody (A12459).
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