Recombinant rabbit monoclonal [ESR1/2299R] antibody to Estrogen Receptor alpha.
Specificity
This antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human Estrogen Receptor alpha protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ESR1/2299R
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
~67 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Estrogen Receptor alpha Antibody [ESR1/2299R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Western Blot - Anti-Estrogen Receptor alpha Antibody [ESR1/2299R] (A248486)
Western blot analysis of human MCF-7 cell lysate using Anti-Estrogen Receptor alpha Antibody [ESR1/2299R].
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