Anti-ESR1 Antibody (A304863)

Name: Anti-ESR1 Antibody
Brand: Antibodies.com
SKU: A304863
Price: 520.0 USD
Availability: InStock

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$520

Rabbit polyclonal antibody to ESR1 for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-ESR1 Antibody

Description

Rabbit polyclonal antibody to ESR1.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human

Immunogen

Synthetic peptide from the mid-protein of Human ESR1.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~66 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ER, ER-alpha, ESR, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunohistochemistry - Anti-ESR1 Antibody (A304863)

Immunohistochemistry analysis of human breast, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-ESR1 Antibody (A304863) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

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Immunohistochemistry - Anti-ESR1 Antibody (A304863)

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Western Blot - Anti-ESR1 Antibody (A304863)

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of ~66.2 kDa ESR1 protein using Anti-ESR1 Antibody (A304863) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~66.2 kDa. Other Band(s): 50 kDa is isoform 2.

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Immunofluorescence - Anti-ESR1 Antibody (A304863)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ESR1 Antibody (A304863), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Nucleus, Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ESR1 Antibody. (D) Composite.

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