Anti-ERp57 Antibody [Map.ERp57] (A305093)

Name: Anti-ERp57 Antibody [Map.ERp57]
Brand: Antibodies.com
SKU: A305093
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [Map.ERp57] antibody to ERp57 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-ERp57 Antibody [Map.ERp57]

Description

Mouse monoclonal [Map.ERp57] antibody to ERp57.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:2,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Monkey, Porcine, Rabbit

Immunogen

Recombinant human ERp57 (Grp58) protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

Map.ERp57

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER protein 57, ER protein 60, ERp60, GRP58, p58, PDIA3, Protein disulfide-isomerase A3

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunofluorescence - Anti-ERp57 Antibody [Map.ERp57] (A305093)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-ERp57 Antibody [Map.ERp57] (A305093), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasmic and perinuclear staining.

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Western Blot - Anti-ERp57 Antibody [Map.ERp57] (A305093)

Western blot analysis of human cell lysates showing detection of Erp57 protein using Anti-ERp57 Antibody [Map.ERp57] (A305093) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-ERp57 Antibody [Map.ERp57] (A305093)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-ERp57 Antibody [Map.ERp57] (A305093) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis and Hair Follicles.

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Immunofluorescence - Anti-ERp57 Antibody [Map.ERp57] (A305093)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-ERp57 Antibody [Map.ERp57] (A305093), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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