Anti-ERp57 Antibody [4F9] (A304860)

Name: Anti-ERp57 Antibody [4F9]
Brand: Antibodies.com
SKU: A304860
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [4F9] antibody to ERp57 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-ERp57 Antibody [4F9]

Description

Mouse monoclonal [4F9] antibody to ERp57.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human

Immunogen

Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged).

Host

Mouse

Clonality

Monoclonal

Clone ID

4F9

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER protein 57, ER protein 60, ERp60, GRP58, p58, PDIA3, Protein disulfide-isomerase A3

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-ERp57 Antibody [4F9] (A304860)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Anti-ERp57 Antibody [4F9] (A304860) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 57 kDa.

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Immunofluorescence - Anti-ERp57 Antibody [4F9] (A304860)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ERp57 Antibody [4F9] (A304860), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.

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