Anti-ERK2 Antibody (A83402)

Name: Anti-ERK2 Antibody
Brand: Antibodies.com
SKU: A83402
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to ERK2 for ELISA, WB and IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-ERK2 Antibody

Description

Goat polyclonal antibody to ERK2.

Applications

ELISA, WB, IF

Dilutions

ELISA: 1:8,000, WB: 0.5-2µg/ml, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human ERK2.

Sequence

CAAGPEMVRGQVF

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

38 kDa

Predicted MW

41.4kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ERK-2, ERT1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, p42-MAPK, PRKM1, PRKM2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-ERK2 Antibody (A83402)

ERK2 expression in HepG2 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ERK2 Antibody (A83402) at 0.5µg/ml and detected by chemiluminescence.

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Immunofluorescence - Anti-ERK2 Antibody (A83402)

ERK2 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK2 Antibody (A83402) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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