Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Name: Anti-ERK1 (phospho Tyr204) Antibody
Brand: Antibodies.com
SKU: A16536
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to ERK1 (phospho Tyr204) for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-ERK1 (phospho Tyr204) Antibody

Description

Rabbit polyclonal antibody to ERK1 (phospho Tyr204).

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y204 of human ERK1 (NP_002737.2).

Sequence

TEYVA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa / 44 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1, p44-MAPK, PRKM3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at 1:1,000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Immunohistochemistry analysis of paraffin-embedded human uterus using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.