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Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody (A16677)

Western Blot - Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody (A16677) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody
Description
Rabbit polyclonal antibody to ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187).
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T185 & Y187 of human ERK2MAPK1 (NP_002736.3).
Sequence
LTEYV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
42 kDa / 44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody (A16677) - Antibodies.comEnlarge Image

Western Blot - Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody (A16677)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-ERK1 (phospho Thr202 + Tyr204) + ERK2 (phospho Thr185 + Tyr187) Antibody (A16677) at 1:500 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
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