Anti-ERK1 Antibody [ARC2591] (A306776)

Name: Anti-ERK1 Antibody [ARC2591]
Brand: Antibodies.com
SKU: A306776
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2591] antibody to ERK1 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-ERK1 Antibody [ARC2591]

Description

Rabbit monoclonal [ARC2591] antibody to ERK1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (P27361).

Sequence

MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2591

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

44 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1, p44-MAPK, PRKM3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-ERK1 Antibody [ARC2591] (A306776)

Western blot analysis of various lysates, using Anti-ERK1 Antibody [ARC2591] (A306776) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-ERK1 Antibody [ARC2591] (A306776)

Western blot analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using Anti-ERK1 Antibody [ARC2591] (A306776) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-ERK1 Antibody [ARC2591] (A306776)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ERK1 Antibody [ARC2591] (A306776) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ERK1 Antibody [ARC2591] (A306776)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ERK1 Antibody [ARC2591] (A306776) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.