Anti-ERK1 Antibody (A305114)

Name: Anti-ERK1 Antibody
Brand: Antibodies.com
SKU: A305114
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to ERK1 for WB, IHC, ICC/IF and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-ERK1 Antibody

Description

Rabbit polyclonal antibody to ERK1.

Applications

WB, IHC, ICC/IF, Flow Cytometry

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100, FCM: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Sheep, Chicken, Drosophila, Xenopus

Immunogen

A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~44 kDa (ERK1) / ~42 kDa (ERK2)

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1, p44-MAPK, PRKM3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Immunofluorescence - Anti-ERK1 Antibody (A305114)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-ERK1 Antibody (A305114), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.

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Immunohistochemistry - Anti-ERK1 Antibody (A305114)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-ERK1 Antibody (A305114) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.

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Immunofluorescence - Anti-ERK1 Antibody (A305114)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-ERK1 Antibody (A305114), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Cytoplasm. Nucleus.

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Immunohistochemistry - Anti-ERK1 Antibody (A305114)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-ERK1 Antibody (A305114) at 1:25,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..