Anti-EpCAM Antibody [EGP40/1372] (HRP) (A277522)

Mouse monoclonal [EGP40/1372] antibody to EpCAM (HRP) for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-EpCAM Antibody [EGP40/1372] (HRP)
Description
Mouse monoclonal [EGP40/1372] antibody to EpCAM (HRP).
Specificity
This antibody is directly conjugated to Horse Radish Peroxidase (HRP) enzyme. Compared to indirect-primary-secondary detection system, it offers the following advantages: 1) Assay time is shorter as only one antibody is involved, 2) Background is reduced as there is no secondary antibody, and 3) It can be used to detect the target protein in the antibody-host samples (i.e. Mouse-on-Mouse or Rabbit-on-Rabbit), avoiding problem related to host-immunoglobulins.Epitope of this MAb is mapped between aa 202-209 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment from the extracellular domain, around amino acids 100-224, of human EpCAM protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
EGP40/1372
Isotype
IgG1
Conjugate

HRP

Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
40-43 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% ProClin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, hEGP314, M1S2, M4S1, MIC18, TACSTD1, TROP1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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