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Anti-EpCAM Antibody [ARC0521] (A306962)

Western Blot - Anti-EpCAM Antibody [ARC0521] (A306962) - Antibodies.com Immunohistochemistry - Anti-EpCAM Antibody [ARC0521] (A306962) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC0521] antibody to EpCAM for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-EpCAM Antibody [ARC0521]
Description
Rabbit monoclonal [ARC0521] antibody to EpCAM.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human EpCAM (P16422).
Sequence
MAPPQVLAFGLLLAAATATFAAAQEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0521
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, hEGP314, M1S2, M4S1, MIC18, TACSTD1, TROP1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-EpCAM Antibody [ARC0521] (A306962) - Antibodies.comEnlarge Image

Western Blot - Anti-EpCAM Antibody [ARC0521] (A306962)

Western blot analysis of extracts of various cell lines, using Anti-EpCAM Antibody [ARC0521] (A306962) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-EpCAM Antibody [ARC0521] (A306962) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-EpCAM Antibody [ARC0521] (A306962)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-EpCAM Antibody [ARC0521] (A306962) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-EpCAM Antibody [ARC0521] (A306962)? Please let us know so that we can list the citation on this page.

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