Rabbit polyclonal antibody to eNOS (Phospho-Thr495)
Specificity
The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII
Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody #11711.The lane on the right is treated with the antigen-specific peptide.
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