Rabbit polyclonal antibody to eNOS (Phospho-Ser615)
Specificity
The antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 615 (F-N-S(p)-I-S) derived from Human eNOS.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody #12137. The lane on the right is treated with the synthesized peptide.
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