Anti-eNOS (phospho Ser1177) Antibody [ARC58017] (A308963)

Name: Anti-eNOS (phospho Ser1177) Antibody [ARC58017]
Brand: Antibodies.com
SKU: A308963
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC58017] antibody to eNOS (phospho Ser1177) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-eNOS (phospho Ser1177) Antibody [ARC58017]

Description

Rabbit monoclonal [ARC58017] antibody to eNOS (phospho Ser1177).

Applications

Dilutions

WB: 1:2,000-1:8,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).

Sequence

TQSFS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58017

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

cNOS, Constitutive NOS, EC-NOS, Endothelial NOS, Nitric oxide synthase 3, Nitric oxide synthase, endothelial, NOS type III, NOS3, NOSIII

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-eNOS (phospho Ser1177) Antibody [ARC58017] (A308963)

Western blot analysis of 293T lysates, using Anti-eNOS (phospho Ser1177) Antibody [ARC58017] (A308963) at 1:7,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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