Anti-Enolase 1 Antibody [253] (A85406)

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat cerebellum section with Anti-Enolase 1 Antibody [253] (A85406) at a dilution of 1:1,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The Anti-Enolase 1 Antibody [253] (A85406) labels the nuclei of non neuronal cells in the molecular and granular cell layers with a concentration at the nuclear membrane. Purkinje and granule neurons are notably negative. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed human and rat tissues.

NIH/3T3 cells stained with Anti-Enolase 1 Antibody (green), Anti-Vimentin Antibody (A85421 | red) and DNA (blue). The Anti-Enolase 1 Antibody reveals strong cytoplasmic staining, while the Anti-Vimentin Antibody reveals cytoplasmic intermediate filaments.

Immunofluorescent analysis of HeLa cells stained with Anti-Enolase 1 Antibody [253] (A85406), at a dilution of 1:500, in green, and co-stained with Anti-Heat Shock Protein 60 Antibody (A85438), at a dilution of 1:5,000, in red. The blue is DAPI staining of nuclear DNA. The Anti-Enolase 1 Antibody [253] (A85406) reveals strong cytoplasmic staining while the Anti-Heat Shock Protein 60 Antibody (A85438) specifically labels mitochondria in these cells.

Western blot analysis of different cell lysates using Anti-Enolase 1 Antibody [253] (A85406), at a dilution of 1:10,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] NIH-3T3 l cells, [Lane 3] C6 cells, [Lane 4] HEK293 cells, [Lane 5] HeLa cells, and [Lane 6] SH-SY5Y cells. A strong single band at 47 kDa corresponds to the Enolase 1 protein.