All Lanes Mouse anti-human ENO2 monoclonal antibody at 1ug/ml. Lane 1 Hela cell lysate. Lane 2 Recombinant Human Gamma-enolase (ENO2) at 10ugLane 3 U251 cell lysate. Secondary Goat polyclonal to Mouse IgG at 1/3000 dilution Predicted band size: 48 kDa. Observed band size: 48kDaAdditional bands at: 100kDa. We are unsure as to the identity of this extra band.