Anti-eIF4E Antibody [ARC0447] (A308059)

Name: Anti-eIF4E Antibody [ARC0447]
Brand: Antibodies.com
SKU: A308059
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0447] antibody to eIF4E for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-eIF4E Antibody [ARC0447]

Description

Rabbit monoclonal [ARC0447] antibody to eIF4E.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:100-1:500

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 118-217 of human eIF4E (P06730).

Sequence

NKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0447

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-eIF4E Antibody [ARC0447] (A308059)

Western blot analysis of extracts of various cell lines, using Anti-eIF4E Antibody [ARC0447] (A308059) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Western Blot - Anti-eIF4E Antibody [ARC0447] (A308059)

Immunoprecipitation analysis of 600µg extracts of mouse testis using 3µg of Anti-eIF4E Antibody [ARC0447] (A308059). This Western blot was performed on the immunoprecipitate using Anti-eIF4E Antibody [ARC0447] (A308059) at a dilution of 1:500.

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