Western blot analysis of various lysates, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.
Western blot analysis of extracts from wild type(WT) and eIF4E knockout (KO) 293T(KO) cells, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-eIF4E Antibody (A13922). This Western blot was performed on the immunoprecipitate using Anti-eIF4E Antibody (A13922) at a dilution of 1:1000.
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