Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Name: Anti-EIF2S1 (phospho Ser51) Antibody
Brand: Antibodies.com
SKU: A89604
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to EIF2S1 (phospho Ser51) for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Ribosomal Marker

Specifications

Name

Anti-EIF2S1 (phospho Ser51) Antibody

Description

Rabbit polyclonal antibody to EIF2S1 (phospho Ser51).

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000-1:2,000, IP: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S51 of human eIF2a (NP_004085.1).

Sequence

SELSR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Western blot analysis of extracts of HeLa cells, using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at 1:2,000 dilution or EIF2S1 antibody (A0764). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Immunofluorescence analysis of HeLa and HeLa treated with CA cells using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-EIF2S1 (phospho Ser51) Antibody (A89604). This Western blot was performed on the immunoprecipitate using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.

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