Western Blot - Anti-EIF2S1 (phospho Ser49) Antibody (A16585)
Western blot analysis of extracts from HeLa cells, using Anti-EIF2S1 (phospho Ser49) Antibody (A16585). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-EIF2S1 (phospho Ser49) Antibody (A16585). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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