Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777)

Name: Anti-EGFR (phospho Y992) Antibody [EM-12]
Brand: Antibodies.com
SKU: A86777
Price: 495.0 USD
Availability: InStock

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$495

Mouse monoclonal [EM-12] antibody to EGFR (phospho Y992) for IP, WB and Flow Cytometry (Intracellular).

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Tags:

Endosomal Marker ER Marker

Specifications

Name

Anti-EGFR (phospho Y992) Antibody [EM-12]

Description

Mouse monoclonal [EM-12] antibody to EGFR (phospho Y992).

Specificity

This antibody reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 992.

Applications

IP, WB, Flow Cytometry (Intracellular)

Dilutions

WB: 1 µg/ml; Positive Control: EGF-stimulated A431, negative control: non-stimulated A431, Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reactivity

Human

Immunogen

Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.

Host

Mouse

Clonality

Monoclonal

Clone ID

EM-12

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunoprecipitation - Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777)

Immunoprecipitation of EGFR from EGF-treated A431 cells by Anti-EGFR (Phospho-Tyr992) Antibody (A86777) (A), Anti-EGFR (Phospho-Tyr1173) Antibody (A86778) (B), a commercial Anti-EGFR Antibody (C), and Anti-EGFR Antibody [mAb108] (D). The precipitates were immunoblotted with Anti-EGFR (Phospho-Tyr992) Antibody (A86777) or Anti-EGFR (Phospho-Tyr1173) Antibody (A86778), and Goat Anti-Mouse IgG (HRP).

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Immunoprecipitation - Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777)

Immunoprecipitation of EGFR from EGF-treated A431 cells by Anti-EGFR (Phospho-Tyr992) Antibody (A86777) (A), Anti-EGFR (Phospho-Tyr1173) Antibody (A86778) (B), a commercial Anti-EGFR Antibody (C), and Anti-EGFR Antibody [mAb108] (D). The precipitates were immunoblotted with a commercial Anti-EGFR Antibody and Goat Anti-Rabbit IgG (HRP).

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Western Blot - Anti-EGFR (phospho Y992) Antibody [EM-12] (A86777)

Western blotting analysis of EGFR (phospho-Tyr992) by Anti-EGFR (Phospho-Tyr992) Antibody (A86777) in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

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