Anti-EGFR (phospho Tyr1197) Antibody (A94203)

$185

Rabbit polyclonal antibody to EGFR (phospho Tyr1197) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Endosomal Marker ER Marker

Specifications

Name

Anti-EGFR (phospho Tyr1197) Antibody

Description

Rabbit polyclonal antibody to EGFR (phospho Tyr1197).

Specificity

This antibody detects endogenous levels of EGFR only when phosphorylated at Tyr1197.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human EGFR around the phosphorylation site of Tyr1197 (amino acids 1161-1210).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

134kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Western Blot - Anti-EGFR (phospho Tyr1197) Antibody (A94203)

Western blot analysis of lysates from A431 cells treated with EGF 40 muM 10' using Anti-EGFR (phospho Tyr1197) Antibody. The left hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Immunohistochemistry - Anti-EGFR (phospho Tyr1197) Antibody (A94203)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-EGFR (phospho Tyr1197) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Western Blot - Anti-EGFR (phospho Tyr1197) Antibody (A94203)

Western blot analysis of various cells using Anti-EGFR (phospho Tyr1197) Antibody.

Western Blot - Anti-EGFR (phospho Tyr1197) Antibody (A94203)

Western blot analysis of A431 cells using Anti-EGFR (phospho Tyr1197) Antibody.

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