Anti-EGFR Antibody [ARC1139] (A305672)

Name: Anti-EGFR Antibody [ARC1139]
Brand: Antibodies.com
SKU: A305672
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1139] antibody to EGFR for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Endosomal Marker ER Marker

Specifications

Name

Anti-EGFR Antibody [ARC1139]

Description

Rabbit monoclonal [ARC1139] antibody to EGFR.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human EGFR (L858R) (P00533).

Sequence

DYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1139

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

175 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epidermal growth factor receptor, ERBB, ERBB1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-EGFR Antibody [ARC1139] (A305672)

Western blot analysis of extracts of Mouse brain cells, using Anti-EGFR Antibody [ARC1139] (A305672) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-EGFR Antibody [ARC1139] (A305672)

Immunohistochemistry analysis of paraffin-embedded human lung adenocarcinoma (egfr-e745-a750del positive sample) using Anti-EGFR Antibody [ARC1139] (A305672) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-EGFR Antibody [ARC1139] (A305672)

Immunohistochemistry analysis of paraffin-embedded human lung adenocarcinoma (egfr-l858r positive sample) using Anti-EGFR Antibody [ARC1139] (A305672) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.