Recombinant rabbit monoclonal [5F3] antibody to EGFP.
Applications
WB, IP
Dilutions
WB 1:1,000, IP: 1:200
Host
Rabbit
Clonality
Monoclonal
Clone ID
5F3
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Western blot analysis of EGFP in 293T cells, transfected with or without EGFP. THe primary antibody used was Anti-EGFP Antibody [5F3], cells expressing EGFP show clear positive band at correct position.
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