Western blot analysis of human HeLa showing detection of 134 kDa EGF protein using Anti-EGF Antibody (A304868) at 1:1,000 for 2 hours at room temperature with shaking. Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: 134 kDa. Other Band(s): 80 kDa, 55 kDa.
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