Anti-EBI3 Antibody [ARC2190] (A305621)

Name: Anti-EBI3 Antibody [ARC2190]
Brand: Antibodies.com
SKU: A305621
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC2190] antibody to EBI3 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-EBI3 Antibody [ARC2190]

Description

Rabbit monoclonal [ARC2190] antibody to EBI3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 130-229 of human EBI3 (Q14213).

Sequence

DPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2190

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

EBV-induced gene 3 protein, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta, IL-27B, IL27B, Interleukin-27 subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-EBI3 Antibody [ARC2190] (A305621)

Western blot analysis of extracts of MCF7 cells, using Anti-EBI3 Antibody [ARC2190] (A305621) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-EBI3 Antibody [ARC2190] (A305621)

Western blot analysis of extracts of various cell lines, using Anti-EBI3 Antibody [ARC2190] (A305621) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-EBI3 Antibody [ARC2190] (A305621)

Immunofluorescence analysis of rat spleen cells using Anti-EBI3 Antibody [ARC2190] (A305621) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-EBI3 Antibody [ARC2190] (A305621)

Immunofluorescence analysis of human spleen cells using Anti-EBI3 Antibody [ARC2190] (A305621) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).